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KMID : 1101420180500020205
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Korean Journal of Clinical Laboratory Science
2018 Volume.50 No. 2 p.205 ~ p.210
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DNA Breakage by Salvianolic acid B in the Presence of Cu (II)
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Lee Pyeong-Jae
Moon Cheol
Choi Yoon-Seon
Son Hyun-Kyu
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Abstract
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Salvianolic acid B, which is a compound in the Salvia miltiorrhiza, has diverse biological activities, In particular, the antioxidative effects were reported to be involved in the protection of hepatocytes, neurons, and various cell types. On the other hand, some phenolic compounds, such as ferulic acid, which is regarded as an antioxidant, plays a pro-oxidative role in the specific transitional metal environment, which could explain the anticancer effect. This study examined the pro-oxidative effects of salvianolic acid B in the presence of Cu2+. Treatment with both salvianolic acid B and Cu2+ induced the transition of supercoiled DNA to the open circular or linear form but not in the sole salvianolic acid B or Cu2+ treatments. Salvianolic acid B reduced the Cu2+ to Cu+ using neocuproine, a Cu+ specific chelator. In addition, catalase, an enzyme that breaks down the H2O2 to water and molecular oxygen, inhibited the DNA breakage. H2O2, a reactive oxygen species, has detrimental effects on biological molecules, particularly DNA. Overall, the reduction of Cu2+ by salvianolic acid B could lead to the production of H2O2 followed by DNA breakage. These results suggest that the pro-oxidative effects could be the one of the anti-cancer mechanisms of salvianolic acid B, which remains to be explained.
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KEYWORD
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Antioxidant, Copper ion, Hydroxyl radical, Pro-oxidant, Salvianolic acid B
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